Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/44292
Title: | Green space exposure and blood DNA methylation at birth and in childhood - A multi-cohort study | Authors: | Aguilar-Lacasana, Sofia Marques, Irene Fontes de Castro, Montserrat Dadvand, Payam Escriba, Xavier Fossati, Serena Gonzalez, Juan R. Nieuwenhuijsen, Mark ALFANO, Rossella Annesi-Maesano, Isabella Brescianini, Sonia Burrows, Kimberley Calas, Lucinda Elhakeem, Ahmed Heude, Barbara Hough, Amy Isaevska, Elena Jaddoe, Vincent W. V. Lawlor, Deborah A. Monaghan, Genevieve Richiardi, Lorenzo NAWROT, Tim Watmuff, Aidan PLUSQUIN, Michelle Yang, Tiffany C. Vrijheid, Martine Felix, Janine F. Bustamante, Mariona |
Issue Date: | 2024 | Publisher: | PERGAMON-ELSEVIER SCIENCE LTD | Source: | Environment international, 188 (Art N° 108684) | Abstract: | Green space exposure has been associated with improved mental, physical and general health. However, the underlying biological mechanisms remain largely unknown. The aim of this study was to investigate the association between green space exposure and cord and child blood DNA methylation. Data from eight European birth cohorts with a total of 2,988 newborns and 1,849 children were used. Two indicators of residential green space exposure were assessed: (i) surrounding greenness (satellite-based Normalized Difference Vegetation Index (NDVI) in buffers of 100 m and 300 m) and (ii) proximity to green space (having a green space >= 5,000 m2 2 within a distance of 300 m). For these indicators we assessed two exposure windows: (i) pregnancy, and (ii) the period from pregnancy to child blood DNA methylation assessment, named as cumulative exposure. DNA methylation was measured with the Illumina 450K or EPIC arrays. To identify differentially methylated positions (DMPs) we fitted robust linear regression models between pregnancy green space exposure and cord blood DNA methylation and between cumulative green space exposure and child blood DNA methylation. Two sensitivity analyses were conducted: (i) without adjusting for cellular composition, and (ii) adjusting for air pollution. Cohort results were combined through fixed-effect inverse variance weighted meta-analyses. Differentially methylated regions (DMRs) were identified from meta-analysed results using the Enmix-combp and DMRcate methods. There was no statistical evidence of pregnancy or cumulative exposures associating with any DMP (False Discovery Rate, FDR, p-value < 0.05). However, surrounding greenness exposure was inversely associated with four DMRs (three in cord blood and one in child blood) annotated to ADAMTS2, KCNQ1DN, SLC6A12 and SDK1 genes. Results did not change substantially in the sensitivity analyses. Overall, we found little evidence of the association between green space exposure and blood DNA methylation. Although we identified associations between surrounding greenness exposure with four DMRs, these findings require replication. | Notes: | Aguilar-Lacasaña, S (corresponding author), ISGlobal, Barcelona, Spain.; Aguilar-Lacasaña, S (corresponding author), Univ Pompeu Fabra UPF, Barcelona, Spain.; Aguilar-Lacasaña, S (corresponding author), CIBER Epidemiol & Salud Publ, Madrid, Spain.; Aguilar-Lacasaña, S (corresponding author), Univ Barcelona, Barcelona, Spain. sofia.aguilar@isglobal.org |
Keywords: | Green space;DNA methylatio;Cord blood;Child blood;DMPDMR | Document URI: | http://hdl.handle.net/1942/44292 | ISSN: | 0160-4120 | e-ISSN: | 1873-6750 | DOI: | 10.1016/j.envint.2024.108684 | ISI #: | 001308044700001 | Datasets of the publication: | https://doi.org/10.5281/zenodo.11058025 | Rights: | 2024 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY-NC license (http://creativecommons.org/licenses/bync/4.0/). | Category: | A1 | Type: | Journal Contribution |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Green space exposure and blood DNA methylation at birth and in childhood – A multi-cohort study.pdf | Published version | 1.14 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.