Interference of extracellular soluble algal organic matter on flocculation-sedimentation harvesting of Chlorella sp.

Abstract

Extracellular soluble algal organic matter (AOM) significantly interferes with microalgae flocculation. This study investigated the effects of various AOM fractions on Chlorella sp. flocculation using ferric chloride (FeCl3), sodium hydroxide (NaOH), and chitosan. All flocculants achieved high separation efficiency (87-99 %), but higher dosages were required in the presence of AOM. High molecular weight (>50 kDa) AOM fraction was identified as the primary inhibitor of flocculation across different pH levels, whereas low/medium molecular weight (<3 and <50 kDa) AOM had minimal impact. Compositional analysis revealed that the inhibitory AOM fraction is a glycoprotein rich in carbohydrates, including neutral, amino, and acidic sugars. The significance of this study is in identifying carboxyl groups (-COOH) from acidic monomers in >50 kDa AOM that inhibit flocculation. Understanding AOM composition and the interaction dynamics between AOM, cells, and flocculants is crucial for enhancing the techno-economics and sustainability of flocculation-based microalgae harvesting.