Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/7967
Title: | Uncertainty of measurement for competitive and indirect ELISAs | Authors: | Toussaint, J.F. ASSAM NKOUIBERT, Pryseley Calj, B. Dekeyser, F. Knapen, K. Imberechts, Hein Goris, Nesya MOLENBERGHS, Geert Mintiens, K. Declercq, Kris |
Issue Date: | 2007 | Publisher: | OFFICE INT EPIZOOTIES | Source: | REVUE SCIENTIFIQUE ET TECHNIQUE-OFFICE INTERNATIONAL DES EPIZOOTIES, 26(3). p. 649-656 | Abstract: | A method for the estimation of the uncertainty of measurements for Gaussian outcomes of enzyme-linked immunosorbent assay (ELISA) is described using competitive and indirect foot and mouth disease (FMD) ELISAs. Assay repeatability was determined by random effects analysis of variance, and the normality of the residuals was checked. The standard errors of the individual predicted values were transformed into confidence intervals around the corresponding observed values and further transformed into probabilities of being above/below a cut-off. Logistic regression models were subsequently used to interpolate probability values for the whole range of possible assay values. The uncertainty of measurement of a test result was finally defined as the probability of not observing the same qualitative test result when retesting the same sample. For the competitive ELISA any sample with a percent inhibition 4% above the cut-off value had an uncertainty level (probability of a negative result in the case of retest) below 5%. In the indirect ELISA with a cut-off OD of 0.1, the uncertainty was below 5% for any sample with a normalised OD value above 0.22. | Notes: | Vet & Agrochem Res Ctr, B-1180 Uccle, Belgium. Univ Hasselt, Ctr Stat, B-3590 Diepenbeek, Belgium.Toussaint, JF, Vet & Agrochem Res Ctr, Groeselenberg 99, B-1180 Uccle, Belgium.krdec@var.fgov.be | Keywords: | solid phase competition ELISA (SPCE); foot and mouth disease; indirect ELISA; logistic regression model; repeatability; uncertainty of measurement;solid phase competition ELISA (SPCE); foot and mouth disease; indirect ELISA; logistic regression model; repeatability; uncertainty of measurement | Document URI: | http://hdl.handle.net/1942/7967 | Link to publication/dataset: | https://www.researchgate.net/publication/5557869_Uncertainty_of_measurement_for_competitive_and_indirect_ELISAs | ISSN: | 0253-1933 | e-ISSN: | 1608-0637 | DOI: | 10.20506/rst.26.3.1775 | ISI #: | 000252724700011 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2009 |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
230-2-9.pdf Restricted Access | Published version | 168.68 kB | Adobe PDF | View/Open Request a copy |
SCOPUSTM
Citations
1
checked on Sep 3, 2020
WEB OF SCIENCETM
Citations
1
checked on Apr 22, 2024
Page view(s)
18
checked on Sep 7, 2022
Download(s)
10
checked on Sep 7, 2022
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.