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Title: | Na-P-i cotransporter type I activity causes a transient intracellular alkalinization during ATP depletion in rabbit medullary thick ascending limb cells | Authors: | JANS, Frank AMELOOT, Marcel WOUTERS, P. STEELS, Paul |
Issue Date: | 2008 | Publisher: | NATL RESEARCH COUNCIL CANADA-N R C RESEARCH PRESS | Source: | CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, 86(1-2). p. 36-45 | Abstract: | The cellular pathophysiology of renal ischemia-reperfusion injury was investigated in primary cell cultures from rabbit medullary thick ascending limb (MTAL). Metabolic inhibition (MI) was achieved with cyanide and 2-deoxy-glucose. Sixty minutes of MI caused a profound but reversible decrease in intracellular concentration of ATP ([ATP](i)). Intracellular pH (pH(i)) first decreased after initiation of MI, followed by a transient alkalinization. When [ATP](i) reached its lowest value (<1% of control), the cells slowly acidified to reach a stable pH(i) of 6.92 after 50 min of MI. In the presence of EIPA (10 mu mol/L), the pattern of changes in pH(i) was unchanged and acidification was not increased, indicating that the Na+/H+ exchangers were inactive during ATP depletion. When inorganic phosphate (P-i) or Na+ was omitted from the apical solutions during MI, the transient alkalinization was no longer observed and the cytosol slowly acidified. Experiments on Na+-dependent alkalinizations revealed the presence of a Na-P-i cotransporter in the apical cell membrane. With indirect immunofluorescence, the Na-P-i cotransporter expressed in these primary cell cultures could be identified as Na-P-i type I. Although the exact physiological role of Na-P-i type I still is unresolved, these experiments demonstrate that apical Na-P-i type I activity is increased at the onset of ATP depletion in MTAL cells. | Notes: | Univ Hasselt, Dept Physiol, Biomed Res Inst, B-3590 Diepenbeek, Belgium. Catholic Univ Louvain, Dept Anaesthesiol, Univ Hosp Gasthuisberg, B-3000 Louvain, Belgium.Jans, F, Univ Hasselt, Dept Physiol, Biomed Res Inst, B-3590 Diepenbeek, Belgium.frank.jans@hotmaii.com | Keywords: | MTAL; cell culture; ischemia; metabolic inhibition; intracellular pH; alkalinization; Na-P-i cotransporter | Document URI: | http://hdl.handle.net/1942/8008 | ISSN: | 0008-4212 | e-ISSN: | 1205-7541 | DOI: | 10.1139/Y07-114 | ISI #: | 000253544900006 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2009 |
Appears in Collections: | Research publications |
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