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Title: | Variability of polymorphic families of three types of xylanase inhibitors in the wheat grain proteome | Authors: | Courtin, CM CROES, Kristof Gebruers, K. ROBBEN, Johan NOBEN, Jean-Paul Samyn, B. Debyser, G. Van Beeumen, J. Delcour, CM |
Issue Date: | 2008 | Publisher: | WILEY-VCH VERLAG GMBH | Source: | PROTEOMICS, 8(8). p. 1692-1705 | Abstract: | Cereals contain proteinaceous inhibitors of endo-beta-1,4-xylanases (E.C.3.2.1.8, xylanases). Since these xylanase inhibitors (XIs) are only active against xylanases of microbial origin and do not interact with plant endogenous xylanases, they are believed to act as a defensive barrier against phytopathogenic attack. So far, three types of XIs have been identified, i.e. Triticum aestivum XI (TAXI), xylanase inhibiting protein (XIP), and thaumatin-like XI (TIXI) proteins. In this study the variation in XI forms present in wheat grain was elucidated using high-resolution 2-DE in combination with LC-ESI-MS/MS and biochemical techniques. Reproducible 2-DE fingerprints of TAXI-, XIP-, and TIXI-type XIs, selectively purified from whole meal of three European wheat cultivars using cation exchange chromatography followed by affinity chromatography, were obtained using a pH-gradient of 6 to 11 and a molecular mass range of 10 to 60 kDa. Large polymorphic XI families, not known to date, which exhibit different pl-and/or molecular mass values, were visualised by colloidal CBB staining. Identification of distinct genetic variants by MS/MS-analysis provides a partial explanation for the observed XI heterogeneity. Besides genetic diversity, PTMs, such as glycosylation, account for the additional complexity of the 2-DE patterns. | Notes: | Katholieke Univ Leuven, Dept Microbial & Mol Syst, Lab Food Chem & Biochem, B-3001 Louvain, Belgium. Hasselt Univ, Biomed Res Inst, Diepenbeek, Belgium. Transnatl Univ Limburg, Sch Life Sci, Diepenbeek, Belgium. Univ Ghent, Lab Protein Biochem & Protein Engn, B-9000 Ghent, Belgium. | Keywords: | polymorphism; wheat; xylanase inhibitors | Document URI: | http://hdl.handle.net/1942/8274 | ISSN: | 1615-9853 | e-ISSN: | 1615-9861 | DOI: | 10.1002/pmic.200700813 | ISI #: | 000255314300014 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2009 |
Appears in Collections: | Research publications |
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variability.pdf | Non Peer-reviewed author version | 1.23 MB | Adobe PDF | View/Open |
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