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Title: | Diffusion of myelin oligodendrocyte glycoprotein in living OLN-93 cells investigated by raster-scanning image correlation spectroscopy (RICS) | Authors: | GIELEN, Ellen SMISDOM, Nick DE CLERCQ, Ben VAN DE VEN, Martin Gijsbers, Rik Debyser, Zeger RIGO, Jean-Michel Hofkens, Johan Engelborghs, Yves AMELOOT, Marcel |
Issue Date: | 2008 | Publisher: | SPRINGER/PLENUM PUBLISHERS | Source: | JOURNAL OF FLUORESCENCE, 18(5). p. 813-819 | Abstract: | Many membrane proteins and lipids are partially confined in substructures ranging from tens of nanometers to micrometers in size. Evidence for heterogeneities in the membrane of oligodendrocytes, i.e. the myelin-producing cells of the central nervous system, is almost exclusively based on detergent methods. However, as application of detergents can alter the membrane phase behaviour, it is important to investigate membrane heterogeneities in living cells. Here, we report on the first investigations of the diffusion behavior of the myelin-specific protein MOG (myelin oligodendrocyte glycoprotein) in OLN-93 as studied by the recently developed RICS (raster-scanning image correlation spectroscopy) technique. We implemented RICS on a standard confocal laser-scanning microscope with one-photon excitation and analog detection. Measurements on FITC-dextran were used to evaluate the performance of the system and the data analysis procedure. | Notes: | Hasselt Univ, Biomed Res Inst, Lab Cell Physiol, B-3590 Diepenbeek, Belgium. Catholic Univ Louvain, Lab Biomol Dynam, B-3001 Heverlee, Belgium. Eindhoven Univ Technol, Dept Appl Phys, NL-5600 MB Eindhoven, Netherlands. Catholic Univ Louvain, Lab Mol Virol & Gene Therapy, B-3000 Louvain, Belgium. Catholic Univ Louvain, Lab Photochem & Spect, B-3001 Heverlee, Belgium. Transnatl Univ Limburg, B-3590 Diepenbeek, Belgium. | Keywords: | raster-scanning image correlation spectroscopy; myelin oligodendrocyte glycoprotein; OLN-93; diffusion; lipid rafts | Document URI: | http://hdl.handle.net/1942/8530 | ISSN: | 1053-0509 | e-ISSN: | 1573-4994 | DOI: | 10.1007/s10895-007-0308-8 | ISI #: | 000259187600007 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2009 |
Appears in Collections: | Research publications |
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Diffusion.pdf | Non Peer-reviewed author version | 495.07 kB | Adobe PDF | View/Open |
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