Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/9254
Title: | On the use of Z-scan fluorescence correlation experiments on giant unilamellar vesicles | Authors: | GIELEN, Ellen VAN DE VEN, Martin Margineanu, Anca Dedecker, Peter Van der Auweraer, Mark Engelborghs, Yves Hofkens, Johan AMELOOT, Marcel |
Issue Date: | 2009 | Publisher: | ELSEVIER SCIENCE BV | Source: | CHEMICAL PHYSICS LETTERS, 469(1-3). p. 110-114 | Abstract: | Z-scan fluorescence correlation spectroscopy (FCS) uses multiple focus planes to determine diffusion coefficients in supported phospholipid bilayers (SPBs) without the need for extrinsic calibration. The approach can be used to discriminate diffusion modes. In this work, Z-scan FCS was applied to study the diffusion of the fluorescent lipid analogue DiI-C-18(5) in the top membrane of giant unilamellar vesicles (GUVs) in the electroformation chamber. In contrast to SPBs, the intrinsic estimate of the radial waist might differ from the one obtained by extrinsic calibration. We describe a pragmatic approach to verify the validity of the Z-scan approach under these conditions. (C) 2008 Elsevier B.V. All rights reserved. | Notes: | [Gielen, Ellen; vandeVen, Martin; Ameloot, Marcel] Hasselt Univ, B-3590 Diepenbeek, Belgium. [Gielen, Ellen; vandeVen, Martin; Ameloot, Marcel] Transnat Univ Limburg, Sch Life Sci, Biomed Res Inst, B-3590 Diepenbeek, Belgium. [Gielen, Ellen; Engelborghs, Yves] Katholieke Univ Leuven, Lab Biomol Dynam, B-3001 Heverlee, Belgium. [Margineanu, Anca] Univ London Imperial Coll Sci Technol & Med, Dept Chem, London SW7 2AZ, England. [Dedecker, Peter; Van der Auweraer, Mark; Hofkens, Johan] Katholieke Univ Leuven, Dept Chem, B-3001 Heverlee, Belgium. | Document URI: | http://hdl.handle.net/1942/9254 | ISSN: | 0009-2614 | e-ISSN: | 1873-4448 | DOI: | 10.1016/j.cplett.2008.12.061 | ISI #: | 000262801400022 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2010 |
Appears in Collections: | Research publications |
Show full item record
SCOPUSTM
Citations
10
checked on Sep 3, 2020
WEB OF SCIENCETM
Citations
11
checked on Oct 13, 2024
Page view(s)
136
checked on Jun 17, 2023
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.