Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/9765
Title: Use of freeze-cracking in ontogenetic research in Macrostomum lignano (Macrostomida, Rhabditophora)
Authors: Willems, Maxime
Boone, Mieke
Couvreur, Marjolein
De Mulder, Katrien
Van Ranst, Jelka
ARTOIS, Tom 
Borgonie, Gaetan
Issue Date: 2009
Publisher: SPRINGER
Source: DEVELOPMENT GENES AND EVOLUTION, 219(5). p. 273-279
Abstract: A method for studying whole mount flatworm embryos based on freeze-cracking of the eggs is described. This method allows successful immunohistological and immunocytological studies of whole mount embryos. It does not require the use of sharpened needles or a microinjection system to puncture the eggshell. Moreover, this method is more practical and less time-consuming than classical puncturing and much cheaper than the use of a microinjection system. The advantages of this method are illustrated by results of several immunolocalisation experiments in the macrostomid flatworm Macrostomum lignano. The optimal procedure and crucial steps for this method are discussed.
Notes: [Willems, Maxime; Boone, Mieke; Couvreur, Marjolein; De Mulder, Katrien; Van Ranst, Jelka; Borgonie, Gaetan] Univ Ghent, Dept Biol, Nematol Sect, B-9000 Ghent, Belgium. [Artois, Tom] Hasselt Univ, Ctr Environm Sci, Res Grp Biodivers Phylogeny & Populat Studies, B-3590 Diepenbeek, Belgium.
Keywords: Platyhelminthes; Freeze-cracking; Embryonic development; Eggshell; Immunohistochemistry
Document URI: http://hdl.handle.net/1942/9765
ISSN: 0949-944X
e-ISSN: 1432-041X
DOI: 10.1007/s00427-009-0284-x
ISI #: 000266372500005
Category: A1
Type: Journal Contribution
Validations: ecoom 2010
Appears in Collections:Research publications

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