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|Title:||Fluorescence anisotropy measurements in solution: Methods and reference materials (IUPAC Technical Report)||Authors:||AMELOOT, Marcel
VAN DE VEN, Martin
Ulises Acuna, A.
|Issue Date:||2013||Publisher:||INT UNION PURE APPLIED CHEMISTRY||Source:||PURE AND APPLIED CHEMISTRY, 85 (3), p. 589-608||Abstract:||After recalling the basic relations relevant to both steady-state and time-resolved fluorescence polarization, it is shown how the values of steady-state polarized intensities recorded experimentally usually need to be corrected for systematic effects and errors, caused by instrumentation and sample properties. A list of selected reference values of steady-state fluorescence anisotropy and polarization is given. Attention is also paid to analysis of time-resolved fluorescence anisotropy data obtained by pulse fluorometry or phase and modulation fluorometry techniques. Recommendations for checking the accuracy of measurements are provided together with a list of selected time-resolved fluorescence anisotropy data as reported in the literature.||Notes:||[Ameloot, Marcel; vandeVen, Martin] Univ Hasselt, BIOMED, B-3590 Diepenbeek, Belgium. [Ulises Acuna, A.] CSIC, Inst Quim Fis Rocasolano, E-28006 Madrid, Spain. [Valeur, Bernard] CNAM, Dept CASER, F-75141 Paris 03, France. [Valeur, Bernard] ENS Cachan, Lab PPSM, F-94235 Cachan, France. email@example.com||Keywords:||Chemistry, Multidisciplinary; anisotropy; fluorescence; IUPAC Analytical Chemistry Division; IUPAC Organic and Biomolecular Chemistry Division; IUPAC Physical and Biophysical Chemistry Division; photoluminescence; polarization; standardization;anisotropy; fluorescence; IUPAC Analytical Chemistry Division; IUPAC Organic and Biomolecular Chemistry Division; IUPAC Physical and Biophysical Chemistry Division; photoluminescence; polarization; standardization||Document URI:||http://hdl.handle.net/1942/14782||ISSN:||0033-4545||e-ISSN:||1365-3075||DOI:||10.1351/PAC-REP-11-11-12||ISI #:||000315186600009||Category:||A1||Type:||Journal Contribution||Validations:||ecoom 2014|
|Appears in Collections:||Research publications|
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