Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/14782
Title: Fluorescence anisotropy measurements in solution: Methods and reference materials (IUPAC Technical Report)
Authors: AMELOOT, Marcel 
VAN DE VEN, Martin 
Ulises Acuna, A.
Valeur, Bernard
Issue Date: 2013
Publisher: INT UNION PURE APPLIED CHEMISTRY
Source: PURE AND APPLIED CHEMISTRY, 85 (3), p. 589-608
Abstract: After recalling the basic relations relevant to both steady-state and time-resolved fluorescence polarization, it is shown how the values of steady-state polarized intensities recorded experimentally usually need to be corrected for systematic effects and errors, caused by instrumentation and sample properties. A list of selected reference values of steady-state fluorescence anisotropy and polarization is given. Attention is also paid to analysis of time-resolved fluorescence anisotropy data obtained by pulse fluorometry or phase and modulation fluorometry techniques. Recommendations for checking the accuracy of measurements are provided together with a list of selected time-resolved fluorescence anisotropy data as reported in the literature.
Notes: [Ameloot, Marcel; vandeVen, Martin] Univ Hasselt, BIOMED, B-3590 Diepenbeek, Belgium. [Ulises Acuna, A.] CSIC, Inst Quim Fis Rocasolano, E-28006 Madrid, Spain. [Valeur, Bernard] CNAM, Dept CASER, F-75141 Paris 03, France. [Valeur, Bernard] ENS Cachan, Lab PPSM, F-94235 Cachan, France. marcel.ameloot@uhasselt.be
Keywords: Chemistry, Multidisciplinary; anisotropy; fluorescence; IUPAC Analytical Chemistry Division; IUPAC Organic and Biomolecular Chemistry Division; IUPAC Physical and Biophysical Chemistry Division; photoluminescence; polarization; standardization;anisotropy; fluorescence; IUPAC Analytical Chemistry Division; IUPAC Organic and Biomolecular Chemistry Division; IUPAC Physical and Biophysical Chemistry Division; photoluminescence; polarization; standardization
Document URI: http://hdl.handle.net/1942/14782
ISSN: 0033-4545
e-ISSN: 1365-3075
DOI: 10.1351/PAC-REP-11-11-12
ISI #: 000315186600009
Category: A1
Type: Journal Contribution
Validations: ecoom 2014
Appears in Collections:Research publications

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