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http://hdl.handle.net/1942/15448
Title: | Cell proliferation monitoring by multiplexed electrochemical impedance spectroscopy on microwell assays | Authors: | DUCHATEAU, Stijn BROEDERS, Jeroen CROUX, Dieter RIGO, Jean-Michel WAGNER, Patrick THOELEN, Ronald DE CEUNINCK, Ward |
Issue Date: | 2013 | Source: | Physica Status Solidi C, 10 (5), p. 882-888 | Series/Report: | Physica Status Solidi C-Current Topics in Solid State Physics | Series/Report no.: | 5 | Abstract: | Cell proliferation can be monitored by a wide range of well-established techniques. Most of the principles used rely on optical, single end-point methods, often involving the use of absorbent, fluorescent or luminescent compounds. These additives can interfere with the cell growth, thus producing distorted results. Electrochemical impedance spectroscopy provides a solution for this problem and enables continuous monitoring without interference. However equipment for this measurement technique is often bulky, highly expensive and lacks multichannel features. This paper presents a low-cost, compact hardware platform optimized for proliferation measurements, together with custom software to ease interpretation and physical modelling of data. Performance is demonstrated and measurement parameters are fine-tuned for three commonly used cell types in proliferation measurements. | Notes: | Duchateau, S (reprint author), Xios Univ Coll, Agoralaan Gebouw H, B-3590 Diepenbeek, Belgium. stijn.duchateau@uhasselt.be | Keywords: | impedance | Document URI: | http://hdl.handle.net/1942/15448 | DOI: | 10.1002/pssc.201200748 | ISI #: | 000322514100038 | Category: | C1 | Type: | Proceedings Paper | Validations: | ecoom 2014 |
Appears in Collections: | Research publications |
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