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http://hdl.handle.net/1942/16666
Title: | Combining Electrochemical Impedance Spectroscopy and Surface Plasmon Resonance into one Simultaneous Read-Out System for the Detection of Surface Interactions | Authors: | VANDENRYT, Thijs Pohl, Andrea VAN GRINSVEN, Bart THOELEN, Ronald DE CEUNINCK, Ward WAGNER, Patrick Opitz, Jörg |
Issue Date: | 2013 | Source: | SENSORS, 13 (11), p. 14650-14661 | Abstract: | In this article we describe the integration of impedance spectroscopy (EIS) and surface plasmon resonance (SPR) into one surface analytic device. A polydimethylsiloxane (PDMS) flow cell is created, matching the dimensions of a commercially available sensor chip used for SPR measurements. This flow cell allowed simultaneous measurements between an EIS and a SPR setup. After a successful integration, a proof of principle study was conducted to investigate any signs of interference between the two systems during a measurement. The flow cell was rinsed with 10 mM Tris-HCl and 1× PBS buffer in an alternating manner, while impedance and shifts of the resonance angle were monitored. After achieving a successful proof of principle, a usability test was conducted. It was assessed whether simultaneous detection occurred when: (i) Protein A is adsorbed to the gold surface of the chip; (ii) The non-occupied zone is blocked with BSA molecules and (iii) IgG1 is bound to the Protein A. The results indicate a successful merge between SPR and EIS. | Keywords: | SPR; EIS; flow-cell; lab on chip; Protein A; immunoglobulin | Document URI: | http://hdl.handle.net/1942/16666 | e-ISSN: | 1424-8220 | DOI: | 10.3390/s131114650 | ISI #: | 000330321100023 | Rights: | © 2013 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/) | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2015 |
Appears in Collections: | Research publications |
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vandenryt 1.pdf | Published version | 694.97 kB | Adobe PDF | View/Open |
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