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Title: Exploring the rhizospheric and endophytic bacterial communities of Acer pseudoplatanus growing on a TNT-contaminated soil: towards the development of a rhizocompetent TNT-detoxifying plant growth promoting consortium
Authors: THIJS, Sofie 
Van Dillewijn, Pieter
SILLEN, Wouter 
TRUYENS, Sascha 
D'HAEN, Jan 
CARLEER, Robert 
AMELOOT, Marcel 
Ramos, Juan-Luis
Issue Date: 2014
Publisher: SPRINGER
Source: PLANT AND SOIL, 385 (1-2), p. 15-36
Abstract: Background and aims Numerous microorganisms have been isolated from trinitrotoluene (TNT)-contaminated soils, however TNT tends to persist, indicating that the microbial biomass or activity is insufficient for degradation. Deep-rooting trees at military sites have been found to take-up contaminants from groundwater, and the extensive root and endosphere provide ideal niches for microbial TNT-transformations. Methods We characterised the rhizosphere, root endosphere and endo-phyllosphere bacteria of Acer pseudoplatanus growing at a historically TNT-contaminated location, using 16S rRNA gene fingerprinting, bacteria isolation, oxidoreductase gene-cloning, in planta growth-promotion (PGP) tests, inoculation, plant physiology measurements and microscopy. Results Based on terminal-restriction-fragment-length-polymorphism analysis, bulk soil and rhizosphere samples were highly clustered. Proteo-and Actinobacteria dominated the rhizosphere and root endosphere, whereas Alphaproteobacteria were more abundant in shoots and Actinobacteria in leaves. We isolated multiple PGP-bacteria and cloned 5 flavin-oxidoreductases belonging to the Old Yellow Enzyme family involved in TNT-reduction from 3 Pseudomonas spp., the leaf symbiont Stenotrophomonas chelatiphaga and the root endophyte Variovorax ginsengisola. Conclusions The inoculation with a selection of these strains, consortium CAP9, which combines efficient TNT-transformation capabilities with beneficial PGP-properties, has the ability to detoxify TNT in the bent grass (Agrostis capillaris) rhizosphere, stimulate plant growth and improve plant health under TNT stress.
Notes: [Thijs, Sofie; Sillen, Wouter; Truyens, Sascha; Holtappels, Michelle; Carleer, Robert; Weyens, Nele; Vangronsveld, Jaco] Hasselt Univ, Ctr Environm Sci, B-3590 Diepenbeek, Belgium. [Van Dillewijn, Pieter] CSIC, Estn Expt Zaidin, E-18008 Granada, Spain. [D'Haen, Jan] IMO, B-3590 Diepenbeek, Belgium. [Ameloot, Marcel] Transnat Univ Limburg, Hasselt Univ, Biomed Res Inst, Sch Life Sci, B-3590 Diepenbeek, Belgium. [Ramos, Juan-Luis] Abengoa Res, E-41014 Seville, Spain.
Keywords: agrostis capillaris; endophyte; OYE, XenB flavin oxidoreductases; plant growth promotion; rhizoremediation; trinitrotoluene;Agrostis capillaris; Endophyte; OYE; XenB flavin oxidoreductases; Plant growth promotion; Rhizoremediation; Trinitrotoluene
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ISSN: 0032-079X
e-ISSN: 1573-5036
DOI: 10.1007/s11104-014-2260-0
ISI #: 000345283400002
Rights: © Springer International Publishing Switzerland 2014
Category: A1
Type: Journal Contribution
Validations: ecoom 2015
Appears in Collections:Research publications

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