Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/21557
Title: | Designing biomedical proteomics experiments: state-of-the-art and future perspectives | Authors: | Maes, Evelyne Kelchtermans, Pieter Bittremieux, Wout De Grave, Kurt Degroeve, Sven HOOYBERGHS, Jef Mertens, Inge Baggerman, Geert Ramon, Jan Laukens, Kris Martens, Lennart VALKENBORG, Dirk |
Issue Date: | 2016 | Publisher: | TAYLOR & FRANCIS LTD | Source: | EXPERT REVIEW OF PROTEOMICS, 13 (5), p. 495-511 | Abstract: | With the current expanded technical capabilities to perform mass spectrometry-based biomedical proteomics experiments, an improved focus on the design of experiments is crucial. As it is clear that ignoring the importance of a good design leads to an unprecedented rate of false discoveries which would poison our results, more and more tools are developed to help researchers designing proteomic experiments. In this review, we apply statistical thinking to go through the entire proteomics workflow for biomarker discovery and validation and relate the considerations that should be made at the level of hypothesis building, technology selection, experimental design and the optimization of the experimental parameters. | Notes: | [Maes, Evelyne; Hooyberghs, Jef; Mertens, Inge; Baggerman, Geert; Valkenborg, Dirk] Vlaamse Instelling Technol Onderzoek, Appl Bio & Mol Syst, Boeretang 200, B-2400 Mol, Belgium. [Maes, Evelyne; Kelchtermans, Pieter; Mertens, Inge; Baggerman, Geert; Valkenborg, Dirk] Univ Antwerp, CFP, B-2020 Antwerp, Belgium. [Kelchtermans, Pieter; Degroeve, Sven; Martens, Lennart] VIB, Ctr Med Biotechnol, Ghent, Belgium. [Kelchtermans, Pieter; Degroeve, Sven; Martens, Lennart] Univ Ghent, Dept Biochem, B-9000 Ghent, Belgium. [Kelchtermans, Pieter; Degroeve, Sven; Martens, Lennart] Univ Ghent, Bioinformat Inst Ghent, B-9000 Ghent, Belgium. [Bittremieux, Wout; Laukens, Kris] Univ Antwerp, Dept Math & Comp Sci, B-2020 Antwerp, Belgium. [Bittremieux, Wout; Laukens, Kris] Univ Antwerp, Univ Antwerp Hosp, Biomed Informat Res Ctr Antwerp Biomina, B-2020 Antwerp, Belgium. [De Grave, Kurt; Ramon, Jan] Katholieke Univ Leuven, Dept Comp Sci, Leuven, Belgium. [Ramon, Jan] INRIA, France, France. [Valkenborg, Dirk] Hasselt Univ, Interuniv Inst Biostat & Stat Bioinformat, Hasselt, Belgium. | Keywords: | Design of experiments; proteomics; mass spectrometry; bioinformatics; computer-aided optimization of LC-MS;design of experiments; proteomics; mass spectrometry; bioinformatics; computer-aided optimization of LC-MS | Document URI: | http://hdl.handle.net/1942/21557 | ISSN: | 1478-9450 | e-ISSN: | 1744-8387 | DOI: | 10.1586/14789450.2016.1172967 | ISI #: | 000375848400006 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2017 |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Manuscript_March2016_revision_final.pdf | Peer-reviewed author version | 248.47 kB | Adobe PDF | View/Open |
SCOPUSTM
Citations
7
checked on Sep 3, 2020
WEB OF SCIENCETM
Citations
9
checked on Oct 12, 2024
Page view(s)
72
checked on Sep 7, 2022
Download(s)
114
checked on Sep 7, 2022
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.