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Title: | Covalent immobilization of DNA on CVD diamond films | Authors: | WENMACKERS, Sylvia HAENEN, Ken NESLADEK, Milos WAGNER, Patrick MICHIELS, Luc VAN DE VEN, Martin AMELOOT, Marcel |
Issue Date: | 2003 | Publisher: | WILEY-V C H VERLAG GMBH | Source: | PHYSICA STATUS SOLIDI A-APPLIED RESEARCH, 199(1). p. 44-48 | Abstract: | Chemical vapour deposited (CVD) diamond is used in DNA immobilization experiments for its high stability and versatility. In this study a double-stranded, 250 base pairs long DNA fragment of the human PKU gene was covalently bound to CVD diamond films. Thymidine was used as a linker molecule for the binding in head-on configuration. The presence of surface-bound FITC-labelled DNA was confirmed by confocal fluorescence microscopy. In denaturation experiments the second, unlabelled strand of each pair was removed resulting in a loss of fluorescence. The possibility of non-specific binding of DNA to diamond can be excluded. (C) 2003 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. | Notes: | Limburgs Univ Centrum, Mat Res Inst, B-3590 Diepenbeek, Belgium. Linburgs Univ Centrum, Biomed Res Inst, B-3590 Diepenbeek, Belgium.Wenmackers, S, Limburgs Univ Centrum, Mat Res Inst, Wetenschapspk 1, B-3590 Diepenbeek, Belgium. | Document URI: | http://hdl.handle.net/1942/2464 | ISSN: | 0031-8965 | e-ISSN: | 1862-6319 | DOI: | 10.1002/pssa.200303822 | ISI #: | 000185422000010 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2004 |
Appears in Collections: | Research publications |
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