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Title: | Selective abdominal venous congestion to investigate cardiorenal interactions in a rat model | Authors: | COPS, Jirka MULLENS, Wilfried VERBRUGGE, Frederik SWENNEN, Quirine Reynders, Carmen PENDERS, Joris RIGO, Jean-Michel HANSEN, Dominique |
Issue Date: | 2018 | Source: | PLoS One, 13(5) (Art N° e0197687) | Abstract: | Abdominal congestion may play an important role in the cardiorenal syndrome and has been demonstrated to drive disease progression. An animal model for abdominal congestion, without other culprit mechanisms that are often present in patients such as low cardiac output or chronic kidney disease, might be interesting to allow a better study of the pathophysiology of the cardiorenal syndrome. The objective of this study was to develop a clinically relevant and valid rat model with abdominal venous congestion and without pre-existing heart and/or kidney dysfunction. To do so, a permanent surgical constriction (20 Gauge) of the thoracic inferior vena cava (IVC) was applied in male Sprague Dawley rats (IVCc, n = 7), which were compared to sham-operated rats (SHAM, n = 6). Twelve weeks after surgery, abdominal venous pressure (mean: 13.8 vs 4.9 mmHg, p < 0.01), plasma creatinine (p < 0.05), plasma cystatin c (p < 0.01), urinary albumin (p < 0.05), glomerular surface area (p < 0.01) and width of Bowman's space (p < 0.05) of the IVCc group were significantly increased compared to the SHAM group for a comparable absolute body weight between groups (559 vs 530g, respectively, p = 0.73). Conventional cardiac echocardiographic and hemodynamic parameters did not differ significantly between both groups, indicating that cardiac function was not compromised by the surgery. In conclusion, we demonstrate that constriction of the thoracic IVC in adult rats is feasible and significantly increases the abdominal venous pressure to a clinically relevant level, thereby inducing abdominal venous congestion. | Notes: | Cops, J (reprint author), Hasselt Univ, Fac Med & Life Sci, REVAL Rehabil Res Ctr, BIOMED Biomed Res Inst, Diepenbeek, Belgium. jirka.cops@uhasselt.be | Document URI: | http://hdl.handle.net/1942/26079 | ISSN: | 1932-6203 | e-ISSN: | 1932-6203 | DOI: | 10.1371/journal.pone.0197687 | ISI #: | 000433521800024 | Rights: | © 2018 Cops et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2019 |
Appears in Collections: | Research publications |
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