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Title: | Single-Shot Detection of Neurotransmitters in Whole-Blood Samples by Means of the Heat-Transfer Method in Combination with Synthetic Receptors | Authors: | VANDENRYT, Thijs VAN GRINSVEN, Bart EERSELS, Kasper Cornelis, Peter Kholwadia, Safira CLEIJ, Thomas THOELEN, Ronald DE CEUNINCK, Ward PEETERS, Marloes WAGNER, Patrick |
Issue Date: | 2017 | Source: | SENSORS, 17(12) (Art N° 2701) | Abstract: | Serotonin is an important neurotransmitter that plays a major role in the pathogenesis of a variety of conditions, including psychiatric disorders. The detection of serotonin typically relies on high-performance liquid chromatography (HPLC), an expensive technique that requires sophisticated equipment and trained personnel, and is not suitable for point-of-care applications. In this contribution, we introduce a novel sensor platform that can measure spiked neurotransmitter concentrations in whole blood samples in a fast and low-cost manner by combining synthetic receptors with a thermal readout technique-the heat-transfer method. In addition, the design of a miniaturized version of the sensing platform is presented that aims to bridge the gap between measurements in a laboratory setting and point-of-care measurements. This fully automated and integrated, user-friendly design features a capillary pumping unit that is compatible with point-of-care sampling techniques such as a blood lancet device (sample volume-between 50 mu L and 300 mu L). Sample pre-treatment is limited to the addition of an anti-coagulant. With this fully integrated setup, it is possible to successfully discriminate serotonin from a competitor neurotransmitter (histamine) in whole blood samples. This is the first demonstration of a point-of-care ready device based on synthetic receptors for the screening of neurotransmitters in complex matrices, illustrating the sensor's potential application in clinical research and diagnosis of e.g., early stage depression. | Notes: | Thoelen, R (reprint author), Hasselt Univ, Inst Mat Res, Wetenschapspk 1, B-3590 Diepenbeek, Belgium. thijs.vandenryt@uhasselt.be; bart.vangrinsven@maastrichtuniversity.nl; kasper.eersels@maastrichtuniversity.nl; peter.cornelis@kuleuven.be; safira.kholwadia@gmail.com; thomas.cleij@maastrichtuniversity.nl; ronald.thoelen@uhasselt.be; ward.deceuninck@uhasselt.be; m.peeters@mmu.ac.uk; patrick.wagner@fys.kuleuven.be | Keywords: | heat-transfermethod; biomimetic sensing; molecularly imprinted polymers; neurotransmitters; point-of-care diagnostics | Document URI: | http://hdl.handle.net/1942/26313 | e-ISSN: | 1424-8220 | DOI: | 10.3390/s17122701 | ISI #: | 000423285800001 | Rights: | © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2019 |
Appears in Collections: | Research publications |
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File | Description | Size | Format | |
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vandenryt 1.pdf | Published version | 3.8 MB | Adobe PDF | View/Open |
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