Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/28635
Title: | ScFv-modified graphene-coated IDE-arrays for 'label-free' screening of cardiovascular disease biomarkers in physiological saline | Authors: | DELLE, Lotta Pachauri, Vivek Sharma, Shikha Shaforost, Olena Ma, Hui Adabi, Mohammad Lilischkis, Rainer WAGNER, Patrick THOELEN, Ronald Klein, Norbert O'Kennedy, Richard Ingebrandt, Sven |
Issue Date: | 2018 | Publisher: | ELSEVIER ADVANCED TECHNOLOGY | Source: | BIOSENSORS & BIOELECTRONICS, 102, p. 574-581 | Abstract: | Fatty-add binding proteins (FABP) and myeloperoxidases (MPO) are associated with many chronic conditions in humans and considered to be important biomarkers for diagnosis of cardiac diseases. Here we assemble a new electrical biosensor platform based on graphene-coated interdigitated electrode arrays (IDE-arrays) towards ultrafast, label-free screening of heart type-FABP and MPO. Arrays of nanoscale (nanoIDE) and microscale (microIDE) electrode-arrays were fabricated on wafer-scale by combining nanoimprint and photolithography processes. Chemical vapor deposition grown multilayer graphene was transferred onto nano/microIDE-arrays and used as a high surface-to-volume ratio electrical transducer. Novel biofunctional layers of specially engineered anti-h-FABP and anti-MPO single-chain fragment variables (scFv) were immobilized onto graphene-coated IDE-array sensor platform for electrical detection of h-FABP and MPO in physiological saline. scFv fragments show increased sensitivity in comparison to the state-of-the-art competitive ELISA for their higher affinity towards target analytes. Deploying FABP and MPO specific scFvs as receptor molecules onto our high sensitivity graphene-coated IDE-arrays with identical sensor characteristics and assays covering clinically relevant concentrations in physiological saline, we demonstrate realization of a simple and versatile biosensor platform capable of high performance cardiac-bioassays for point-of-care applications. | Notes: | [Delle, Lotta E.; Pachauri, Vivek; Lilischkis, Rainer; Ingebrandt, Sven] Univ Appl Sci Kaiserslautern, Dept Informat & Microsyst Technol, Amerikastr 10, D-66482 Zweibrucken, Germany. [Sharma, Shikha; Ma, Hui; O'Kennedy, Richard] Dublin City Univ, Sch Biotechnol, Dublin 9, Ireland. [Shaforost, Olena; Adabi, Mohammad; Klein, Norbert] Imperial Coll London, Dept Mat, London SW7 2AZ, England. [Wagner, Patrick] Katholieke Univ Leuven, Dept Phys & Astron, Soft Matter Phys & Biophys Sect, Celestijnenlaan 200d, B-3001 Leuven, Belgium. [Thoelen, Ronald] Hassell Univ, Inst Mat Res, Wetenschapspk 1, B-3590 Diepenbeek, Belgium. | Keywords: | scFV; Graphene; Biosensor; Cardiovascular; Electrical sensing; Physiological buffer;scFV; Graphene; Biosensor; Cardiovascular; Electrical sensing; Physiological buffer | Document URI: | http://hdl.handle.net/1942/28635 | ISSN: | 0956-5663 | e-ISSN: | 1873-4235 | DOI: | 10.1016/j.bios.2017.12.005 | ISI #: | 000424176600076 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2019 |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
1-s2.0-S0956566317308011-main.pdf Restricted Access | Published version | 1.13 MB | Adobe PDF | View/Open Request a copy |
s1-ln251118795844769-1939656818Hwf-1569681356IdV18329150802511187PDF_HI0001.pdf | Peer-reviewed author version | 1.61 MB | Adobe PDF | View/Open |
SCOPUSTM
Citations
7
checked on Sep 2, 2020
WEB OF SCIENCETM
Citations
19
checked on Apr 22, 2024
Page view(s)
104
checked on Sep 5, 2022
Download(s)
224
checked on Sep 5, 2022
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.