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Title: | ScFv-modified graphene-coated IDE-arrays for 'label-free' screening of cardiovascular disease biomarkers in physiological saline | Authors: | DELLE, Lotta Pachauri, Vivek Sharma, Shikha Shaforost, Olena Ma, Hui Adabi, Mohammad Lilischkis, Rainer WAGNER, Patrick THOELEN, Ronald Klein, Norbert O'Kennedy, Richard Ingebrandt, Sven |
Issue Date: | 2018 | Publisher: | ELSEVIER ADVANCED TECHNOLOGY | Source: | BIOSENSORS & BIOELECTRONICS, 102, p. 574-581 | Abstract: | Fatty-add binding proteins (FABP) and myeloperoxidases (MPO) are associated with many chronic conditions in humans and considered to be important biomarkers for diagnosis of cardiac diseases. Here we assemble a new electrical biosensor platform based on graphene-coated interdigitated electrode arrays (IDE-arrays) towards ultrafast, label-free screening of heart type-FABP and MPO. Arrays of nanoscale (nanoIDE) and microscale (microIDE) electrode-arrays were fabricated on wafer-scale by combining nanoimprint and photolithography processes. Chemical vapor deposition grown multilayer graphene was transferred onto nano/microIDE-arrays and used as a high surface-to-volume ratio electrical transducer. Novel biofunctional layers of specially engineered anti-h-FABP and anti-MPO single-chain fragment variables (scFv) were immobilized onto graphene-coated IDE-array sensor platform for electrical detection of h-FABP and MPO in physiological saline. scFv fragments show increased sensitivity in comparison to the state-of-the-art competitive ELISA for their higher affinity towards target analytes. Deploying FABP and MPO specific scFvs as receptor molecules onto our high sensitivity graphene-coated IDE-arrays with identical sensor characteristics and assays covering clinically relevant concentrations in physiological saline, we demonstrate realization of a simple and versatile biosensor platform capable of high performance cardiac-bioassays for point-of-care applications. | Notes: | [Delle, Lotta E.; Pachauri, Vivek; Lilischkis, Rainer; Ingebrandt, Sven] Univ Appl Sci Kaiserslautern, Dept Informat & Microsyst Technol, Amerikastr 10, D-66482 Zweibrucken, Germany. [Sharma, Shikha; Ma, Hui; O'Kennedy, Richard] Dublin City Univ, Sch Biotechnol, Dublin 9, Ireland. [Shaforost, Olena; Adabi, Mohammad; Klein, Norbert] Imperial Coll London, Dept Mat, London SW7 2AZ, England. [Wagner, Patrick] Katholieke Univ Leuven, Dept Phys & Astron, Soft Matter Phys & Biophys Sect, Celestijnenlaan 200d, B-3001 Leuven, Belgium. [Thoelen, Ronald] Hassell Univ, Inst Mat Res, Wetenschapspk 1, B-3590 Diepenbeek, Belgium. | Keywords: | scFV; Graphene; Biosensor; Cardiovascular; Electrical sensing; Physiological buffer;scFV; Graphene; Biosensor; Cardiovascular; Electrical sensing; Physiological buffer | Document URI: | http://hdl.handle.net/1942/28635 | ISSN: | 0956-5663 | e-ISSN: | 1873-4235 | DOI: | 10.1016/j.bios.2017.12.005 | ISI #: | 000424176600076 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2019 |
Appears in Collections: | Research publications |
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1-s2.0-S0956566317308011-main.pdf Restricted Access | Published version | 1.13 MB | Adobe PDF | View/Open Request a copy |
s1-ln251118795844769-1939656818Hwf-1569681356IdV18329150802511187PDF_HI0001.pdf | Peer-reviewed author version | 1.61 MB | Adobe PDF | View/Open |
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