Accelerated Polypeptide Synthesis via N ‐Carboxyanhydride Ring Opening Polymerization in Continuous Flow

Abstract

In nature, polypeptide-based materials are ubiquitous, yet their synthetic production is hampered by high cost, limited scalability, and often stringent reaction conditions. Herein an elegant approach is presented forN-carboxyanhydride ring opening polymerization (NCA ROP) ofN epsilon-benzyloxycarbonyl-l-lysine (ZLL) and gamma-benzyl-l-glutamate (BLG) NCA in continuous flow. The polymerization is initiated by primary amine initiators usingN,N-dimethylformamide (DMF) as solvent. Carrying out the reaction in a silicon microflow reactor speeds up the rate of ROP (92% conversion in 40 min in flow as opposed to 6 h in batch) due to highly efficient permeation of CO(2)through the reactor tubing. The polymerization strategy provides a facile, scale-up friendly alternative to traditional batch mode polymerization and has the capability of streamlining NCA ROP.