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Title: | The gut brain in a dish: Murine primary enteric nervous system cell cultures | Authors: | Schonkeren, SL Kuthe, TT Idris, M BON FRAUCHES OLIVEIRA, Ana Carina BOESMANS, Werend Melotte, V |
Issue Date: | 2022 | Publisher: | WILEY | Source: | Neurogastroenterology and motility (Print), 34 (2) (Art N° e14215) | Abstract: | Background The enteric nervous system (ENS) is an extensive neural network embedded in the wall of the gastrointestinal tract that regulates digestive function and gastrointestinal homeostasis. The ENS consists of two main cell types; enteric neurons and enteric glial cells. In vitro techniques allow simplified investigation of ENS function, and different culture methods have been developed over the years helping to understand the role of ENS cells in health and disease. Purpose This review focuses on summarizing and comparing available culture protocols for the generation of primary ENS cells from adult mice, including dissection of intestinal segments, enzymatic digestions, surface coatings, and culture media. In addition, the potential of human ENS cultures is also discussed. | Keywords: | adult mouse;enteric glial cells;enteric neurons;primary ENS culture;protocol | Document URI: | http://hdl.handle.net/1942/36087 | ISSN: | 1350-1925 | e-ISSN: | 1365-2982 | DOI: | 10.1111/nmo.14215 | ISI #: | 000670601900001 | Rights: | 2021 The Authors. Neurogastroenterology & Motility published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2022 |
Appears in Collections: | Research publications |
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Neurogastroenterology Motil - 2021 - Schonkeren - The gut brain in a dish Murine primary enteric nervous system cell.pdf | Published version | 1.3 MB | Adobe PDF | View/Open |
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