Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/37699
Title: | Methylome-wide analysis of IVF neonates that underwent embryo culture in different media revealed no significant differences | Authors: | Koeck, Rebekka M. Busato, Florence Tost, Jorg Consten, Dimitri Van Echten-Arends, Jannie Mastenbroek, Sebastiaan Wurth, Yvonne Remy, Sylvie LANGIE, Sabine NAWROT, Tim PLUSQUIN, Michelle ALFANO, Rossella BIJNENS, Esmee Gielen , Marij van Golde, Ron Dumoulin, John C. M. Brunner, Han van Montfoort, Aafke P. A. Esteki, Masoud Zamani |
Issue Date: | 2022 | Publisher: | NATURE PORTFOLIO | Source: | npj Genomic Medicine, 7 (1) (Art N° 39) | Abstract: | A growing number of children born are conceived through in vitro fertilisation (IVF), which has been linked to an increased risk of adverse perinatal outcomes, as well as altered growth profiles and cardiometabolic differences in the resultant individuals. Some of these outcomes have also been shown to be influenced by the use of different IVF culture media and this effect is hypothesised to be mediated epigenetically, e.g. through the methylome. As such, we profiled the umbilical cord blood methylome of IVF neonates that underwent preimplantation embryo development in two different IVF culture media (G5 or HTF), using the Infinium Human Methylation EPIC BeadChip. We found no significant methylation differences between the two groups in terms of: (i) systematic differences at CpG sites or regions, (ii) imprinted sites/genes or birth weight-associated sites, (iii) stochastic differences presenting as DNA methylation outliers or differentially variable sites, and (iv) epigenetic gestational age acceleration. | Notes: | Esteki, MZ (corresponding author), Maastricht Univ, Med Ctr, Dept Clin Genet, Maastricht, Netherlands.; Esteki, MZ (corresponding author), Maastricht Univ, Med Ctr, Dept Epidemiol, Maastricht, Netherlands.; Esteki, MZ (corresponding author), Maastricht Univ, Med Ctr, Nutr & Toxicol Res Inst Maastricht NUTRIM, Maastricht, Netherlands.; van Montfoort, APA (corresponding author), Maastricht Univ, Med Ctr, GROW Sch Oncol & Reprod, Dept Obstet & Gynaecol, Maastricht, Netherlands. aafke.van.montfoort@mumc.nl; masoud.zamaniesteki@mumc.nl |
Document URI: | http://hdl.handle.net/1942/37699 | e-ISSN: | 2056-7944 | DOI: | 10.1038/s41525-022-00310-3 | ISI #: | WOS:000818814800001 | Rights: | The Author(s) 2022. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http:// creativecommons.org/licenses/by/4.0/. | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2023 |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Methylome-wide analysis of IVF neonates that underwent embryo culture in different media revealed no significant differences.pdf | Published version | 1.52 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.