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Title: | Synchronized, Spontaneous, and Oscillatory Detachment of Eukaryotic Cells: A New Tool for Cell Characterization and Identification | Authors: | Yongabi, Derick KHORSHID, Mehran LOSADA-PEREZ, Patricia Sichani, Soroush Bakhshi Jooken , Stijn Stilman, Wouter Thesseling, Florian Martens, Tobie Van Thillo , Toon Verstrepen, Kevin Dedecker , Peter Vanden Berghe, Pieter Lettinga, Minne Paul Bartic, Carmen Lieberzeit, Peter Schoning, Michael J. THOELEN, Ronald Fransen, Marc Wubbenhorst, Michael WAGNER, Patrick |
Issue Date: | 2022 | Publisher: | WILEY | Source: | Advanced science (Weinheim), (Art N° 2200459) | Status: | Early view | Abstract: | Despite the importance of cell characterization and identification for diagnostic and therapeutic applications, developing fast and label-free methods without (bio)-chemical markers or surface-engineered receptors remains challenging. Here, we exploit the natural cellular response to mild thermal stimuli and propose a label- and receptor-free method for fast and facile cell characterization. Cell suspensions in a dedicated sensor are exposed to a temperature gradient, which stimulates synchronized and spontaneous cell-detachment with sharply defined time-patterns, a phenomenon unknown from literature. These patterns depend on metabolic activity (controlled through temperature, nutrients, and drugs) and provide a library of cell-type-specific indicators, allowing to distinguish several yeast strains as well as cancer cells. Under specific conditions, synchronized glycolytic-type oscillations are observed during detachment of mammalian and yeast-cell ensembles, providing additional cell-specific signatures. These findings suggest potential applications for cell viability analysis and for assessing the collective response of cancer cells to drugs. | Notes: | Yongabi, D; Wagner, P (corresponding author), Katholieke Univ Leuven, Dept Phys & Astron, Lab Soft Matter & Biophys, Celestijnenlaan 200 D, B-3001 Leuven, Belgium. derick.yongabi@kuleuven.be; patrickhermann.wagner@kuleuven.be |
Keywords: | cancer therapy;cell characterization;cell detection;glycolytic oscillations;heat-transfer method;metabolic activity;spontaneous cell detachment | Document URI: | http://hdl.handle.net/1942/37795 | e-ISSN: | 2198-3844 | DOI: | 10.1002/advs.202200459 | ISI #: | 000819977500001 | Rights: | 2022 The Authors. Advanced Science published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2023 |
Appears in Collections: | Research publications |
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Synchronized, Spontaneous, and Oscillatory Detachment of Eukaryotic Cells_ A New Tool for Cell Characterization and Identification.pdf | Published version | 5.45 MB | Adobe PDF | View/Open |
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