Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/42628
Title: | Use of DNA thermodynamics for low-abundance mutation detection by DNA hybridization | Authors: | STULENS, Yannick VAN HOOF, Rebekka MUELLER, Brittany Hollanders, Karen Jacobs, An Kolpashchikov, Dmitry Nelissen, Inge WAGNER, Patrick HOOYBERGHS, Jef |
Advisors: | Hooyberghs | Issue Date: | 2023 | Source: | Abstract: | Mutation detection is of great importance in many biological processes and diagnostics. Single-nucleotide variations are common, where a single base-pair is substituted during replication of wild-type DNA. Duplex formation via hybridisation provides an important detection technique where one of the strands is designed to match the mutant sequence and probe its presence. The probe is, however, subject to cross-hybridisation of wild-type DNA, lowering detection sensitivity in case of low-abundant mutants in a majority wild-type background. We use thermodynamics-based probe design and Langmuir theory to increase sensitivity and quantify the amount of mutant in a sample. | Document URI: | http://hdl.handle.net/1942/42628 | Category: | C2 | Type: | Conference Material |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
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YannickStulens_BPS_Abstract.pdf | Conference material | 525.96 kB | Adobe PDF | View/Open |
YannickStulens_BPS2023.pdf | Conference material | 8.45 MB | Adobe PDF | View/Open |
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