DNA attachment to nanocrystalline diamond films

Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/924

Title:	DNA attachment to nanocrystalline diamond films
Authors:	WENMACKERS, Sylvia CHRISTIAENS, Pieter DAENEN, Michael HAENEN, Ken NESLADEK, Milos VAN DE VEN, Martin VERMEEREN, Veronique MICHIELS, Luc AMELOOT, Marcel WAGNER, Patrick
Issue Date:	2005
Publisher:	Wiley
Source:	Physica Status Solidi (a), 202(11). p. 2212-2216
Abstract:	A biochemical method to immobilize DNA on synthetic diamond for biosensor applications is developed. Nanocrystalline diamond is grown using microwave plasma-enhanced chemical vapour deposition. On the hydrogen-terminated surface 10-undecenoic acid is tethered photochemically under 254 nm illumination, followed by 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide crosslinker-mediated attachment of amino modified DNA. The attachment is functionally confirmed by comparison of supernatant fluorescence and gel electrophoresis. The linking procedure allowed for 35 denaturation and rehybridisation steps.
Keywords:	Biosensors
Document URI:	http://hdl.handle.net/1942/924
ISSN:	0031-8965
e-ISSN:	1862-6319
DOI:	10.1002/pssa.200561932
ISI #:	000231925500031
Category:	A1
Type:	Journal Contribution
Validations:	ecoom 2006
Appears in Collections:	Research publications

Check