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Title: Imaging viral infection: studies on Nicotiana benthamiana plants infected with the pepper mild mottle tobamovirus
Authors: Pérez-Bueno, M.
CISCATO, Massimo 
VAN DE VEN, Martin 
Garcia-Luque, I.
VALCKE, Roland 
Barón, M.
Issue Date: 2006
Publisher: Springer
Source: PHOTOSYNTHESIS RESEARCH, 90(2). p. 111-123
Abstract: We have studied by kinetic Chl-fluorescence imaging (Chl-FI) Nicotiana benthamiana plants infected with the Italian strain of the pepper mild mottle tobamovirus (PMMoV-I). We have mapped leaf photosynthesis at different points of the fluorescence induction curve as well as at different post-infection times. Images of different fluorescence parameters were obtained to investigate which one could discriminate control from infected leaves in the absence of symptoms. The non-photochemical quenching (NPQ) of excess energy in photosystem II (PSII) seems to be the most adequate chlorophyll fluorescence parameter to assess the effect of tobamoviral infection on the chloroplast. Non-symptomatic mature leaves from inoculated plants displayed a very characteristic time-varying NPQ pattern. In addition, a correlation between NPQ amplification and virus localization by tissue-print was found, suggesting that an increase in the local NPQ values is associated with the areas invaded by the pathogen. Changes in chloroplast ultrastructure in non-symptomatic leaf areas showing different NPQ levels were also investigated. A gradient of ultrastructural modifications was observed among the different areas.
Keywords: Biotic stress; Chlorophyll fluorescence imaging; Non-photochemical quenching; Pepper mild mottle virus; Photosystem II; Tobamovirus
Document URI:
ISSN: 0166-8595
e-ISSN: 1573-5079
DOI: 10.1007/s11120-006-9098-0
ISI #: 000243623900003
Category: A1
Type: Journal Contribution
Validations: ecoom 2008
Appears in Collections:Research publications

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