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|Title:||Imaging viral infection: studies on Nicotiana benthamiana plants infected with the pepper mild mottle tobamovirus||Authors:||Pérez-Bueno, M.
VAN DE VEN, Martin
|Issue Date:||2006||Publisher:||Springer||Source:||PHOTOSYNTHESIS RESEARCH, 90(2). p. 111-123||Abstract:||We have studied by kinetic Chl-fluorescence imaging (Chl-FI) Nicotiana benthamiana plants infected with the Italian strain of the pepper mild mottle tobamovirus (PMMoV-I). We have mapped leaf photosynthesis at different points of the fluorescence induction curve as well as at different post-infection times. Images of different fluorescence parameters were obtained to investigate which one could discriminate control from infected leaves in the absence of symptoms. The non-photochemical quenching (NPQ) of excess energy in photosystem II (PSII) seems to be the most adequate chlorophyll fluorescence parameter to assess the effect of tobamoviral infection on the chloroplast. Non-symptomatic mature leaves from inoculated plants displayed a very characteristic time-varying NPQ pattern. In addition, a correlation between NPQ amplification and virus localization by tissue-print was found, suggesting that an increase in the local NPQ values is associated with the areas invaded by the pathogen. Changes in chloroplast ultrastructure in non-symptomatic leaf areas showing different NPQ levels were also investigated. A gradient of ultrastructural modifications was observed among the different areas.||Keywords:||Biotic stress; Chlorophyll fluorescence imaging; Non-photochemical quenching; Pepper mild mottle virus; Photosystem II; Tobamovirus||Document URI:||http://hdl.handle.net/1942/1603||ISSN:||0166-8595||e-ISSN:||1573-5079||DOI:||10.1007/s11120-006-9098-0||ISI #:||000243623900003||Category:||A1||Type:||Journal Contribution||Validations:||ecoom 2008|
|Appears in Collections:||Research publications|
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