Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/1603
Title: Imaging viral infection: studies on Nicotiana benthamiana plants infected with the pepper mild mottle tobamovirus
Authors: Pérez-Bueno, M.
CISCATO, Massimo 
VAN DE VEN, Martin 
Garcia-Luque, I.
VALCKE, Roland 
Barón, M.
Issue Date: 2006
Publisher: Springer
Source: PHOTOSYNTHESIS RESEARCH, 90(2). p. 111-123
Abstract: We have studied by kinetic Chl-fluorescence imaging (Chl-FI) Nicotiana benthamiana plants infected with the Italian strain of the pepper mild mottle tobamovirus (PMMoV-I). We have mapped leaf photosynthesis at different points of the fluorescence induction curve as well as at different post-infection times. Images of different fluorescence parameters were obtained to investigate which one could discriminate control from infected leaves in the absence of symptoms. The non-photochemical quenching (NPQ) of excess energy in photosystem II (PSII) seems to be the most adequate chlorophyll fluorescence parameter to assess the effect of tobamoviral infection on the chloroplast. Non-symptomatic mature leaves from inoculated plants displayed a very characteristic time-varying NPQ pattern. In addition, a correlation between NPQ amplification and virus localization by tissue-print was found, suggesting that an increase in the local NPQ values is associated with the areas invaded by the pathogen. Changes in chloroplast ultrastructure in non-symptomatic leaf areas showing different NPQ levels were also investigated. A gradient of ultrastructural modifications was observed among the different areas.
Keywords: Biotic stress; Chlorophyll fluorescence imaging; Non-photochemical quenching; Pepper mild mottle virus; Photosystem II; Tobamovirus
Document URI: http://hdl.handle.net/1942/1603
ISSN: 0166-8595
e-ISSN: 1573-5079
DOI: 10.1007/s11120-006-9098-0
ISI #: 000243623900003
Category: A1
Type: Journal Contribution
Validations: ecoom 2008
Appears in Collections:Research publications

Show full item record

SCOPUSTM   
Citations

56
checked on Sep 2, 2020

WEB OF SCIENCETM
Citations

59
checked on May 22, 2022

Page view(s)

76
checked on May 24, 2022

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.