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http://hdl.handle.net/1942/1603
Title: | Imaging viral infection: studies on Nicotiana benthamiana plants infected with the pepper mild mottle tobamovirus | Authors: | Pérez-Bueno, M. CISCATO, Massimo VAN DE VEN, Martin Garcia-Luque, I. VALCKE, Roland Barón, M. |
Issue Date: | 2006 | Publisher: | Springer | Source: | PHOTOSYNTHESIS RESEARCH, 90(2). p. 111-123 | Abstract: | We have studied by kinetic Chl-fluorescence imaging (Chl-FI) Nicotiana benthamiana plants infected with the Italian strain of the pepper mild mottle tobamovirus (PMMoV-I). We have mapped leaf photosynthesis at different points of the fluorescence induction curve as well as at different post-infection times. Images of different fluorescence parameters were obtained to investigate which one could discriminate control from infected leaves in the absence of symptoms. The non-photochemical quenching (NPQ) of excess energy in photosystem II (PSII) seems to be the most adequate chlorophyll fluorescence parameter to assess the effect of tobamoviral infection on the chloroplast. Non-symptomatic mature leaves from inoculated plants displayed a very characteristic time-varying NPQ pattern. In addition, a correlation between NPQ amplification and virus localization by tissue-print was found, suggesting that an increase in the local NPQ values is associated with the areas invaded by the pathogen. Changes in chloroplast ultrastructure in non-symptomatic leaf areas showing different NPQ levels were also investigated. A gradient of ultrastructural modifications was observed among the different areas. | Keywords: | Biotic stress; Chlorophyll fluorescence imaging; Non-photochemical quenching; Pepper mild mottle virus; Photosystem II; Tobamovirus | Document URI: | http://hdl.handle.net/1942/1603 | ISSN: | 0166-8595 | e-ISSN: | 1573-5079 | DOI: | 10.1007/s11120-006-9098-0 | ISI #: | 000243623900003 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2008 |
Appears in Collections: | Research publications |
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