Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/17686
Title: Heat transfer resistance as a tool to quantify hybridization efficiency of DNA on a nanocrystalline diamond surface
Authors: Cornelis, P.
VANDENRYT, Thijs 
WACKERS, Gideon 
KELLENS, Evelien 
LOSADA-PEREZ, Patricia 
THOELEN, Ronald 
DE CEUNINCK, Ward 
EERSELS, Kasper 
DRIJKONINGEN, Sien 
HAENEN, Ken 
PEETERS, Marloes 
VAN GRINSVEN, Bart 
WAGNER, Patrick 
Issue Date: 2014
Publisher: ELSEVIER SCIENCE SA
Source: DIAMOND AND RELATED MATERIALS, 48, p. 32-36
Abstract: In this article, we report on a label-free real-time method based on heat transfer resistivity for thermal monitoring of DNA denaturation and its potential to quantify DNA fragments with a specific sequence of interest. Probe DNA, consisting of a 36-mer fragment was covalently immobilized on a nanocrystalline diamond surface, created by chemical vapor deposition on a silicon substrate. Various concentrations of full matched 29-mer target DNA fragments were hybridized with this probe DNA. We observed that the change in heat transfer resistance upon denaturation depends on the concentration of target DNA used during the hybridization, which allowed us to determine the dose-response curve. Therefore, these results illustrate the potential of this technique to quantify the concentration of a specific DNA fragment and to quantify the hybridization efficiency to its probe. (C) 2014 Elsevier B.V. All rights reserved.
Notes: [Cornelis, P.; Vandenryt, T.; Wackers, G.; Kellens, E.; Losada-Perez, P.; Thoelen, R.; De Ceuninck, W.; Eersels, K.; Drijkoningen, S.; Haenen, K.; Peeters, M.; van Grinsven, B.; Wagner, P.] Hasselt Univ, Inst Mat Res, B-3590 Diepenbeek, Belgium. [Vandenryt, T.] XIOS Univ Coll, Dept Appl Engn, B-3590 Diepenbeek, Belgium. [Kellens, E.; Thoelen, R.; De Ceuninck, W.; Peeters, M.; Wagner, P.] IMOMEC, B-3590 Diepenbeek, Belgium. [van Grinsven, B.] Maastricht Univ, Maastricht Sci Programme, NL-6200 MD Maastricht, Netherlands.
Keywords: CVD diamond; heat transfer resistance; DNA quantification; hybridization efficiency analysis;CVD diamond; Heat transfer resistance; DNA quantification; Hybridization efficiency analysis
Document URI: http://hdl.handle.net/1942/17686
ISSN: 0925-9635
e-ISSN: 1879-0062
DOI: 10.1016/j.diamond.2014.06.008
ISI #: 000341900700005
Rights: © 2014 Elsevier B.V. All rights reserved.
Category: A1
Type: Journal Contribution
Validations: ecoom 2015
Appears in Collections:Research publications

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