Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/21569
Title: | Mast cells promote scar remodeling and functional recovery after spinal cord injury via mouse mast cell protease 6 | Authors: | VANGANSEWINKEL, Tim GEURTS, Nathalie Quanten, Kirsten NELISSEN, Sofie LEMMENS, Stefanie GEBOES, Lies DOOLEY, Dearbhaile VIDAL VERA, Pia Pejler, Gunnar HENDRIX, Sven |
Issue Date: | 2016 | Publisher: | FEDERATION AMER SOC EXP BIOL | Source: | FASEB JOURNAL, 30 (5), p. 2040-2057 | Abstract: | An important barrier for axon regeneration and recovery after traumatic spinal cord injury (SCI) is attributed to the scar that is formed at the lesion site. Here, we investigated the effect of mouse mast cell protease (mMCP) 6, a mast cell (MC)-specific tryptase, on scarring and functional recovery after a spinal cord hemisection injury. Functional recovery was significantly impaired in both MC-deficient and mMCP6-knockout (mMCP6(-/-)) mice after SCI compared with wild-type control mice. This decrease in locomotor performance was associated with an increased lesion size and excessive scarring at the injury site. Axon growth-inhibitory chondroitin sulfate proteoglycans and the extracellular matrix components fibronectin, laminin, and collagen IV were significantly up-regulated in MC-deficient and mMCP6(-/-) mice, with an increase in scar volume between 23 and 32%. A degradation assay revealed that mMCP6 directly cleaves fibronectin and collagen IV in vitro. In addition, gene expression levels of the scar components fibronectin, aggrecan, and collagen IV were increased up to 6.8-fold in mMCP6(-/-) mice in the subacute phase after injury. These data indicate that endogenous mMCP6 has scar-suppressing properties after SCI via indirect cleavage of axon growth-inhibitory scar components and alteration of the gene expression profile of these factors.-Vangansewinkel, T., Geurts, N., Quanten, K., Nelissen, S., Lemmens, S., Geboes, L., Dooley, D., Vidal, P. M., Pejler, G., Hendrix, S. Mast cells promote scar remodeling and functional recovery after spinal cord injury via mouse mast cell protease 6. www.fasebj.org | Notes: | [Vangansewinkel, Tim; Geurts, Nathalie; Quanten, Kirsten; Nelissen, Sofie; Lemmens, Stefanie; Geboes, Lies; Dooley, Dearbhaile; Vidal, Pia M.; Hendrix, Sven] Hasselt Univ, Biomed Res Inst, Dept Morphol, Diepenbeek, Belgium. [Pejler, Gunnar] Swedish Univ Agr Sci, Dept Anat Physiol & Biochem, Uppsala, Sweden. [Pejler, Gunnar] Uppsala Univ, Dept Med Biochem & Microbiol, Uppsala, Sweden. [Vidal, Pia M.] Univ Hlth Network, Div Genet & Dev, Krembil Neurosci Ctr, Toronto, ON, Canada. | Keywords: | CNS trauma; mMCP6; ECM remodeling; gene expression; improved outcome;CNS trauma; mMCP6; ECM remodeling; gene expression; improved outcome | Document URI: | http://hdl.handle.net/1942/21569 | ISSN: | 0892-6638 | e-ISSN: | 1530-6860 | DOI: | 10.1096/fj.201500114R | ISI #: | 000374879400030 | Rights: | © FASEB | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2017 |
Appears in Collections: | PhD theses Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
vangansewinkel2016.pdf Restricted Access | Published version | 2.15 MB | Adobe PDF | View/Open Request a copy |
SCOPUSTM
Citations
10
checked on Sep 3, 2020
WEB OF SCIENCETM
Citations
25
checked on Oct 14, 2024
Page view(s)
66
checked on Sep 5, 2022
Download(s)
48
checked on Sep 5, 2022
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.