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http://hdl.handle.net/1942/9828
Title: | Synthetic diamond films as a platform material for label-free protein sensors | Authors: | BIJNENS, Nathalie VERMEEREN, Veronique DAENEN, Michael GRIETEN, Lars HAENEN, Ken WENMACKERS, Sylvia WILLIAMS, Oliver AMELOOT, Marcel VAN DE VEN, Martin MICHIELS, Luc WAGNER, Patrick |
Issue Date: | 2009 | Publisher: | WILEY-V C H VERLAG GMBH, PO BOX 10 11 61, D-69451 WEINHEIM, GERMANY | Source: | PHYSICA STATUS SOLIDI A-APPLICATIONS AND MATERIALS SCIENCE, 206(3). p. 520-526 | Abstract: | In the framework of developing a fast and label-free immunosensor for C-reactive protein (CRP) detection, H-terminated nanocrystalline diamond (NCD) was functionalised with anti-CRP antibodies that were physically adsorbed to the surface. Impedance spectroscopy was used to electronically detect real-time CRP recognition. Different impedance behaviours were observed after CRP addition as compared to after FITC-labelled ssDNA addition at low (100 Hz) as well as at high frequencies (1 MHz). Physical interpretations of the observed impedance changes were obtained by fitting the data to an equivalent electrical circuit. Concentrations of 1 mu M CRP were recognised with a reaction time of 30 minutes. | Keywords: | C-REACTIVE PROTEIN; NANOCRYSTALLINE DIAMOND; IMPEDIMETRIC IMMUNOSENSORS; IMPEDANCE SPECTROSCOPY; DNA HYBRIDIZATION; THIN-FILMS; SURFACES; ELECTRODES; BIOSENSORS; SPR | Document URI: | http://hdl.handle.net/1942/9828 | ISSN: | 1862-6300 | e-ISSN: | 1862-6319 | DOI: | 10.1002/pssa.200880485 | ISI #: | 000264691800018 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2010 |
Appears in Collections: | Research publications |
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