Chinese hamster ovary cell viability on hydrogen and oxygen terminated nano- and microcrystalline diamond surfaces

Abstract

Transfected Chinese hamster ovary cells were cultured on bare uncoated chemical vapor deposited thin nano- and microcrystalline diamond surfaces, hydrophobic hydrogenand hydrophilic oxygen-terminated. Optical and biochemical analyses show that compared to glass controls, growth and viability were not significantly affected (one-way-analysis of variance, ANOVA). Based on two-way ANOVA analyses, neither grain size nor surface termination had a significant influence until 5 days post-seeding.