Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/16795
Title: Mast cells protect from post-traumatic spinal cord damage in mice by degrading inflammation-associated cytokines via mouse mast cell protease 4
Authors: NELISSEN, Sofie 
VANGANSEWINKEL, Tim 
GEURTS, Nathalie 
GEBOES, Lies 
LEMMENS, Evi 
VIDAL VERA, Pia 
LEMMENS, Stefanie 
WILLEMS, Leen 
Boato, Francesco
DOOLEY, Dearbhaile 
Pehl, Debora
Pejler, Gunnar
Maurer, Marcus
Metz, Martin
HENDRIX, Sven 
Issue Date: 2014
Source: NEUROBIOLOGY OF DISEASE, 62, p. 260-272
Abstract: Mast cells (MCs) are found abundantly in the central nervous system and play a complex role in neuroinflammatory diseases such as multiple sclerosis and stroke. In the present study,we show thatMC-deficient KitW-sh/W-sh mice display significantly increased astrogliosis and T cell infiltration as well as significantly reduced functional recovery after spinal cord injury compared to wildtype mice. In addition, MC-deficient mice show significantly increased levels of MCP-1, TNF-α, IL-10 and IL-13 protein levels in the spinal cord.Mice deficient inmouse mast cell protease 4 (mMCP4), an MC-specific chymase, also showed increased MCP-1, IL-6 and IL-13 protein levels in spinal cord samples and a decreased functional outcome after spinal cord injury. A degradation assay using supernatant from MCs derived from either mMCP4−/− mice or controls revealed that mMCP4 cleaves MCP-1, IL-6, and IL-13 suggesting a protective role for MC proteases in neuroinflammation. These data show for the first time that MCs may be protective after spinal cord injury and that they may reduce CNS damage by degrading inflammation-associated cytokines via the MC-specific chymasemMCP4.
Keywords: mMCP4; mast cell; inflammation; spinal cord injury; MCP-1; TNF-α; IL-6; IL-10; IL-13
Document URI: http://hdl.handle.net/1942/16795
ISSN: 0969-9961
e-ISSN: 1095-953X
DOI: 10.1016/j.nbd.2013.09.012
ISI #: 000330553600024
Rights: © 2013 The Authors. Published by Elsevier Inc. All rights reserved. Open access under CC BY license.Open access under CC BY license.
Category: A1
Type: Journal Contribution
Validations: ecoom 2015
Appears in Collections:Research publications

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